Proteintech, 68068-1-Ig, Ferritin light chain Monoclonal antibody

Size: 20ul / 150ul The Ferritin light chain (68068-1-Ig) by Proteintech is a Monoclonal antibody targeting Ferritin light chain in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse samples 68068-1-Ig targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, human placenta tissue Positive IHC detected in: mouse brain tissue, human prostate cancer tissue, human liver cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed. Specification Tested Reactivity: Human, Mouse Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20637 Product name: Recombinant human FTL protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-175 aa of BC008439 Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD Predict reactive species Full Name: ferritin, light polypeptide Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 18-20 kDa GenBank Accession Number: BC008439 Gene Symbol: Ferritin light chain Gene ID (NCBI): 2512 RRID: AB_2918809 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P02792 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.