Proteintech, 68076-1-Ig, BAG3 Monoclonal antibody

Size: 20ul / 150ul The BAG3 (68076-1-Ig) by Proteintech is a Monoclonal antibody targeting BAG3 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68076-1-Ig targets BAG3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: NIH/3T3 cells, LNCaP cells, rabbit heart tissue, HeLa cells, pig heart, rat heart Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17608 Product name: Recombinant human BAG3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 275-575 aa of BC006418 Sequence: SREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP Predict reactive species Full Name: BCL2-associated athanogene 3 Calculated Molecular Weight: 61 kDa Observed Molecular Weight: 74 kDa GenBank Accession Number: BC006418 Gene Symbol: BAG3 Gene ID (NCBI): 9531 RRID: AB_2918814 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O95817 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.