Proteintech, 68091-1-Ig, BNIP3 Monoclonal antibody

Size: 20ul / 150ul The BNIP3 (68091-1-Ig) by Proteintech is a Monoclonal antibody targeting BNIP3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 68091-1-Ig targets BNIP3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells Positive IHC detected in: mouse brain tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Cobalt Chloride treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27935 Product name: Recombinant human BNIP3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-194 aa of BC009342 Sequence: MQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF Predict reactive species Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3 Calculated Molecular Weight: 194 aa, 22 kDa Observed Molecular Weight: 25-30,50-60 kDa GenBank Accession Number: BC009342 Gene Symbol: BNIP3 Gene ID (NCBI): 664 RRID: AB_2918828 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q12983 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.