Proteintech, 68131-1-Ig, MYDGF/C19orf10 Monoclonal antibody

Size: 20ul / 150ul The MYDGF/C19orf10 (68131-1-Ig) by Proteintech is a Monoclonal antibody targeting MYDGF/C19orf10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 68131-1-Ig targets MYDGF/C19orf10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13438 Product name: Recombinant human C19orf10 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-173 aa of BC010129 Sequence: MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL Predict reactive species Full Name: chromosome 19 open reading frame 10 Calculated Molecular Weight: 173 aa, 19 kDa Observed Molecular Weight: 15-19 kDa GenBank Accession Number: BC010129 Gene Symbol: MYDGF/C19orf10 Gene ID (NCBI): 56005 RRID: AB_2923658 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q969H8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.