Product Description
Size: 20ul / 150ul
The FITC (68132-1-Ig) by Proteintech is a Monoclonal antibody targeting FITC in WB, ELISA applications with reactivity to chemical compound, fitc samples
68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with chemical compound, fitc samples.
Tested Applications
Positive WB detected in: FITC-TDP43, FITC-Ag1231, BSA
Positive ELISA detected in: FITC conjugated antibody
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA): ELISA :
Background Information
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer).
This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.
Specification
Tested Reactivity: chemical compound, fitc
Cited Reactivity: mouse
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Immunogen: Peptide Predict reactive species
Full Name: FITC
RRID: AB_2923659
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924