Proteintech, 68133-1-Ig, PLA2G4A Monoclonal antibody

Size: 20ul / 150ul The PLA2G4A (68133-1-Ig) by Proteintech is a Monoclonal antibody targeting PLA2G4A in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 68133-1-Ig targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells, MJ cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165). Specification Tested Reactivity: human, mouse Cited Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag32674 Product name: Recombinant human PLA2G4A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC114340 Sequence: MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFS Predict reactive species Full Name: phospholipase A2, group IVA (cytosolic, calcium-dependent) Calculated Molecular Weight: 749 aa, 85 kDa Observed Molecular Weight: 85-100 kDa GenBank Accession Number: BC114340 Gene Symbol: PLA2G4A Gene ID (NCBI): 5321 RRID: AB_2923660 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P47712 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.