Proteintech, 68154-1-Ig, DDAH2 Monoclonal antibody

Size: 20ul / 150ul The DDAH2 (68154-1-Ig) by Proteintech is a Monoclonal antibody targeting DDAH2 in WB, ELISA applications with reactivity to human, mouse, rat, rabbit samples 68154-1-Ig targets DDAH2 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: rabbit brain tissue, MCF-7 cells, mouse retina tissue, rat colon tissue, rat brain tissue, mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells. Specification Tested Reactivity: human, mouse, rat, rabbit Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7127 Product name: Recombinant human DDAH2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-285 aa of BC001435 Sequence: MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGGPRTVVAGSSDAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLRPGLPGVPPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKAGAGLSSLCLVLSTRPHS Predict reactive species Full Name: dimethylarginine dimethylaminohydrolase 2 Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC001435 Gene Symbol: DDAH2 Gene ID (NCBI): 23564 RRID: AB_2923677 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95865 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.