Proteintech, 68162-1-Ig, FH Monoclonal antibody

Size: 20ul / 150ul The FH (68162-1-Ig) by Proteintech is a Monoclonal antibody targeting FH in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68162-1-Ig targets FH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: A431 cells, HepG2 cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue Positive IHC detected in: human stomach cancer tissue, human placenta tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30254 Product name: Recombinant human FH protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 204-510 aa of BC003108 Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK Predict reactive species Full Name: fumarate hydratase Calculated Molecular Weight: 510 aa, 55 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC003108 Gene Symbol: FH Gene ID (NCBI): 2271 RRID: AB_2923681 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07954 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.