Product Description
Size: 20ul / 150ul
The PPA1 (68178-1-Ig) by Proteintech is a Monoclonal antibody targeting PPA1 in WB, IF/ICC, ELISA applications with reactivity to Human, Rat samples
68178-1-Ig targets PPA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.
Tested Applications
Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).
Specification
Tested Reactivity: Human, Rat
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag7049 Product name: Recombinant human PPA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-289 aa of BC001022 Sequence: MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN Predict reactive species
Full Name: pyrophosphatase (inorganic) 1
Calculated Molecular Weight: 33 kDa
Observed Molecular Weight: 33 kDa
GenBank Accession Number: BC001022
Gene Symbol: PPA1
Gene ID (NCBI): 5464
RRID: AB_2935269
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein G purification
UNIPROT ID: Q15181
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
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