Proteintech, 68252-1-Ig, CNTN1 Monoclonal antibody

Size: 20ul / 150ul The CNTN1 (68252-1-Ig) by Proteintech is a Monoclonal antibody targeting CNTN1 in WB, ELISA applications with reactivity to human, pig, rabbit samples 68252-1-Ig targets CNTN1 in WB, ELISA applications and shows reactivity with human, pig, rabbit samples. Tested Applications Positive WB detected in: A549 cells, pig cerebellum tissue, pig brain tissue, rabbit brain tissue, rabbit cerebellum tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914). Specification Tested Reactivity: human, pig, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5030 Product name: Recombinant human CNTN1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 26-351 aa of BC036569 Sequence: RRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWP Predict reactive species Full Name: contactin 1 Calculated Molecular Weight: 1018 aa, 113 kDa Observed Molecular Weight: 130-135 kDa GenBank Accession Number: BC036569 Gene Symbol: CNTN1 Gene ID (NCBI): 1272 RRID: AB_2935337 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q12860 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.