Proteintech, 68281-1-Ig, LZTFL1 Monoclonal antibody

Size: 20ul / 150ul The LZTFL1 (68281-1-Ig) by Proteintech is a Monoclonal antibody targeting LZTFL1 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 68281-1-Ig targets LZTFL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: Jurkat cells, A431 cells Positive FC (Intra) detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986) Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10733 Product name: Recombinant human LZTFL1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-299 aa of BC025988 Sequence: MAELGLNEHHQNEVINYMRFARSKRGLRLKTVDSCFQDLKESRLVEDTFTIDEVSEVLNGLQAVVHSEVESELINTAYTNVLLLRQLFAQAEKWYLKLQTDISELENQELLEQVAEFEKAEITSSNKKPILDVTKPKLAPLNEGGTAELLNEEILRLQEENEKLKSRLKTIEIQATNALDEKSKLEKALQDLQLDQGNQKDFIKAQDLSNLENTVAALKSEFQKTLNDKTENQKSLEENLATAKHDLLRVREQLHMAEKELEKKFQQTAAYRNMKEILTKKNDQIKDLRKRLAQYEPED Predict reactive species Full Name: leucine zipper transcription factor-like 1 Calculated Molecular Weight: 299 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC025988 Gene Symbol: LZTFL1 Gene ID (NCBI): 54585 RRID: AB_2935362 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NQ48 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.