Proteintech, 68295-1-Ig, Cytokeratin 5/6 Monoclonal antibody

Size: 20ul / 150ul The Cytokeratin 5/6 (68295-1-Ig) by Proteintech is a Monoclonal antibody targeting Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 68295-1-Ig targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue Positive IHC detected in: human tonsillitis tissue, human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human cervical cancer tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:5000-1:20000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: monkey Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11288 Product name: Recombinant human KRT6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 214-564 aa of BC034535 Sequence: FEQYINNLRRQLDNIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQITAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGSSTIKYTTTSSSSRKSYKH Predict reactive species Full Name: keratin 6B Calculated Molecular Weight: 564 aa, 60 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC034535 Gene Symbol: Cytokeratin 6B Gene ID (NCBI): 3854 RRID: AB_2935375 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P04259 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.