Proteintech, 68302-1-Ig, m7G Monoclonal antibody

Size: 20ul / 150ul The m7G (68302-1-Ig) by Proteintech is a Monoclonal antibody targeting m7G in IHC, Dot Blot, ELISA applications with reactivity to chemical compound samples 68302-1-Ig targets m7G in IHC, Dot Blot, ELISA applications and shows reactivity with chemical compound samples. Tested Applications Positive IHC detected in: mouse testis tissue, human colon cancer tissue, human liver cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive ELISA detected in: compound Positive Dot Blot detected in: HeLa cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100 DOT BLOT: DOT BLOT : 1:2500-1:10000 Background Information 7-Methylguanosine (m7G) is a modified purine nucleoside. It is a methylated version of guanosine and when found in human urine, it may be a biomarker of some types of cancer. In the RNAs, 7-methylguanosine have been used to study and examine the reaction evolving methylguanosine. It also plays a role in mRNA as a blocking group at its 5´-end. The m7G modification actively participates in biological and pathological functions by affecting the metabolism of various RNA molecules, including messenger RNA, ribosomal RNA, microRNA, and transfer RNA. Increasing evidence indicates a critical role for m7G in human disease development, especially cancer, and aberrant m7G levels are closely associated with tumorigenesis and progression via regulation of the expression of multiple oncogenes and tumor suppressor genes.Protocol for Dot Blot:https://www.ptglab.com/protocol/68302-1-IgDotBlot.pdf Specification Tested Reactivity: chemical compound Cited Reactivity: mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: m7G RRID: AB_2935381 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.