Proteintech, 68345-1-Ig, Histone H3 Monoclonal antibody

Size: 150ul The Histone H3 (68345-1-Ig) by Proteintech is a Monoclonal antibody targeting Histone H3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, wheat samples 68345-1-Ig targets Histone H3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, wheat samples. Tested Applications Positive WB detected in: LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, MDCK cells, CHO cells, HeLa cells, chicken brain tissue, zebrafish, wheat whole plant Positive IP detected in: HeLa cells Positive IHC detected in: mouse testis tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, A431 cells, HeLa cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Specification Tested Reactivity: human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, wheat Cited Reactivity: human, mouse, rat, pig, canine Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15-17 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_3086558 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.