Proteintech, 68357-1-Ig, DCTD Monoclonal antibody

Size: 20ul / 150ul The DCTD (68357-1-Ig) by Proteintech is a Monoclonal antibody targeting DCTD in WB, IF/ICC, ELISA applications with reactivity to Human samples 68357-1-Ig targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: A549 cells, U2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492). Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10245 Product name: Recombinant human DCTD protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-178 aa of BC001286 Sequence: MSEVSCKKRDDYLEWPEYFMAVAFLSAQRSKDPNSQVGACIVNSENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYPYVCHAELNAIMNKNLTDVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSDKYHDSDEATAARLLFNMAGVTFRKFIPKCSKIVIDFDSINSRPSQKLQ Predict reactive species Full Name: dCMP deaminase Calculated Molecular Weight: 178 aa, 20 kDa Observed Molecular Weight: 18-20 kDa GenBank Accession Number: BC001286 Gene Symbol: DCTD Gene ID (NCBI): 1635 RRID: AB_3085079 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P32321 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.