Proteintech, 68380-1-Ig, Tuberin/TSC2 Monoclonal antibody

Size: 20ul / 150ul The Tuberin/TSC2 (68380-1-Ig) by Proteintech is a Monoclonal antibody targeting Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 68380-1-Ig targets Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, MCF-7 cells, PC-3 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IHC detected in: rat brain tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tuberous sclerosis 2 Calculated Molecular Weight: 1807 aa, 201 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC150300 Gene Symbol: TSC2 Gene ID (NCBI): 7249 RRID: AB_3085098 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P49815 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.