Proteintech, 68431-1-Ig, VASP Monoclonal antibody

Size: 20ul / 150ul The VASP (68431-1-Ig) by Proteintech is a Monoclonal antibody targeting VASP in WB, ELISA applications with reactivity to Human samples 68431-1-Ig targets VASP in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: U2OS cells, A549 cells, A431 cells, MCF-7 cells, HeLa cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP. Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17679 Product name: Recombinant human VASP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-380 aa of BC038224 Sequence: MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP Predict reactive species Full Name: vasodilator-stimulated phosphoprotein Calculated Molecular Weight: 380 aa, 40 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC038224 Gene Symbol: VASP Gene ID (NCBI): 7408 RRID: AB_3085145 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P50552 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.