Proteintech, 68443-1-Ig, VPS4B Monoclonal antibody

Size: 20ul / 150ul The VPS4B (68443-1-Ig) by Proteintech is a Monoclonal antibody targeting VPS4B in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 68443-1-Ig targets VPS4B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. (PMID:31930723) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11610 Product name: Recombinant human VPS4B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-444 aa of BC039574 Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG Predict reactive species Full Name: vacuolar protein sorting 4 homolog B (S. cerevisiae) Calculated Molecular Weight: 444 aa, 49 kDa Observed Molecular Weight: ~50 kDa GenBank Accession Number: BC039574 Gene Symbol: VPS4B Gene ID (NCBI): 9525 RRID: AB_3085156 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75351 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.