Proteintech, 68558-1-Ig, HELIOS Monoclonal antibody

Size: 20ul / 150ul The HELIOS (68558-1-Ig) by Proteintech is a Monoclonal antibody targeting HELIOS in WB, IP, ELISA applications with reactivity to Human samples 68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: Jurkat cells, MOLT-4 cells Positive IP detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4357 Product name: Recombinant human IKZF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 227-526 aa of BC028936 Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH Predict reactive species Full Name: IKAROS family zinc finger 2 (Helios) Calculated Molecular Weight: 500 aa, 57 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC028936 Gene Symbol: HELIOS Gene ID (NCBI): 22807 RRID: AB_3085260 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UKS7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.