Proteintech, 68657-1-Ig, CARD9 Monoclonal antibody

Size: 20ul / 150ul The CARD9 (68657-1-Ig) by Proteintech is a Monoclonal antibody targeting CARD9 in WB, IF/ICC, ELISA applications with reactivity to human samples 68657-1-Ig targets CARD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: NK-92 cells, HL-60 cells, TF-1 cells, THP-1 cells, U-937 cells, MOLT-4 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33643 Product name: Recombinant human CARD9 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 193-492 aa of BC008877 Sequence: SEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGPAGLPGIGAVC Predict reactive species Full Name: caspase recruitment domain family, member 9 Calculated Molecular Weight: 62 kDa Observed Molecular Weight: 55-65 kDa GenBank Accession Number: BC008877 Gene Symbol: CARD9 Gene ID (NCBI): 64170 RRID: AB_3670399 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9H257 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.