Proteintech, 68714-1-Ig, MUC20 Monoclonal antibody

Size: 20ul / 150ul The MUC20 (68714-1-Ig) by Proteintech is a Monoclonal antibody targeting MUC20 in WB, IF/ICC, ELISA applications with reactivity to human, canine samples 68714-1-Ig targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples. Tested Applications Positive WB detected in: MDCK cells, HEK-293 cells, HK-2 cells, A549 cells, SK-BR-3 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953). Specification Tested Reactivity: human, canine Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag31840 Product name: Recombinant human MUC20 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 255-555 aa of BC029267 Sequence: TLLAEALVTVTNIEVINCSITEIETTTSSIPGASDTDPIPTEGVKASSTSDPPALPDSTEAKPHITEVTASAETLSTAGTTESAAPDATIGTPLPTNSTIEREVTAPGATTLSGALATGNPLEETSALSVETPSYVKVSGAAPVSIEAGSAVGKTTSFAGSSASSYSPSEAALKNFTPSETLTTDIATKGPFPTSRDPLPSVPPTTTNSSRGTNSTLAKITTSAKTTMKPPTATPTTARTRPTTDMSAGENGGFLLLRLSVASPEDLTDPRVAERLMQQLHRELHAHAPHFQVSLLRVRRG Predict reactive species Full Name: mucin 20, cell surface associated Calculated Molecular Weight: 55 kDa, 71 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC029267 Gene Symbol: MUC20 Gene ID (NCBI): 200958 RRID: AB_3670407 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8N307 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.