Product Description
Size: 20ul / 150ul
The AP1M1 (68726-1-Ig) by Proteintech is a Monoclonal antibody targeting AP1M1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples
68726-1-Ig targets AP1M1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: MDA-MB-231 cells, A375 cells, NIH/3T3 cells, human placenta tissue, LNCaP cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Specification
Tested Reactivity: human, mouse
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag25303 Product name: Recombinant human AP1M1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 164-423 aa of BC017469 Sequence: IKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ Predict reactive species
Full Name: adaptor-related protein complex 1, mu 1 subunit
Calculated Molecular Weight: 423 aa, 49 kDa
Observed Molecular Weight: 47-49 kDa
GenBank Accession Number: BC017469
Gene Symbol: AP1M1
Gene ID (NCBI): 8907
RRID: AB_3670418
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
UNIPROT ID: Q9BXS5
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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