Proteintech, 68773-1-Ig, Cleaved Caspase 3/P17/P19 Monoclonal antibody

Size: 20ul / 150ul The Cleaved Caspase 3/P17/P19 (68773-1-Ig) by Proteintech is a Monoclonal antibody targeting Cleaved Caspase 3/P17/P19 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 68773-1-Ig targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, A2780 cells, BV-2 cells, HepG2 cells, HSC-T6 cells, Ramos cells Positive IP detected in: Staurosporine treated Jurkat cells Positive IHC detected in: human lymphoma tissue, Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse liver tissue Positive IF/ICC detected in: 1 μM Staurosporine (1/3/6 hour) treated Jurkat cells, 50 μM Cisplatin (18 hours) treated BV-2 cells, 1 μM Staurosporine (1/3/6 hour) treated HSC-T6 cells Positive FC (Intra) detected in: 1 μM Staurosporine (3 hours) treated Jurkat cells, 50 μM Cisplatin（18h）treated BV-2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 3, apoptosis-related cysteine peptidase Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 17 kDa, 19 kDa GenBank Accession Number: NM_032991 Gene Symbol: Caspase 3 Gene ID (NCBI): 836 RRID: AB_3665444 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P42574 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.