Proteintech, 68905-5-Ig, PMS2 Monoclonal antibody

Size: 20ul / 150ul The PMS2 (68905-5-Ig) by Proteintech is a Monoclonal antibody targeting PMS2 in WB, IHC, IP, ELISA applications with reactivity to human, rabbit samples 68905-5-Ig targets PMS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, rabbit samples. Tested Applications Positive WB detected in: LNCaP cells, rabbit brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human tonsillitis tissue, Human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). Specification Tested Reactivity: human, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag34237 Product name: Recombinant human PMS2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-400 aa of BC093921 Sequence: MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDINVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADLEKPMVEKQ Predict reactive species Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae) Calculated Molecular Weight: 862 aa, 96 kDa Observed Molecular Weight: 117 kDa, 100 kDa GenBank Accession Number: BC093921 Gene Symbol: PMS2 Gene ID (NCBI): 5395 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P54278 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.