Proteintech, 80035-2-RR, Phospho-Beta Catenin (Ser552) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Beta Catenin (Ser552) (80035-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-Beta Catenin (Ser552) in WB, ELISA applications with reactivity to human, mouse samples 80035-2-RR targets Phospho-Beta Catenin (Ser552) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Forskolin treated HeLa cells, Forskolin treated A431 cells, Forskolin treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers(PMID: 22617422, PMID: 18334222). Both AMPK and AKT phosphorylates β-catenin at Ser 552. The phosphorylation of β-catenin at Ser552 can stabilize β-catenin, enhance its nuclear accumulation and increase its transcriptional activity(PMID: 2865606, PMID: 20361929). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: catenin (cadherin-associated protein), beta 1, 88kDa Calculated Molecular Weight: 781 aa, 86 kDa Observed Molecular Weight: 86-100 kDa GenBank Accession Number: BC058926 Gene Symbol: Beta Catenin Gene ID (NCBI): 1499 ENSEMBL Gene ID: ENSG00000168036 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35222 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.