Proteintech, 80056-2-RR, Chk1 Recombinant monoclonal antibody

Size: 20ul / 100ul The Chk1 (80056-2-RR) by Proteintech is a Recombinant antibody targeting Chk1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 80056-2-RR targets Chk1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells Positive IF/ICC detected in: HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: CHK1 checkpoint homolog (S. pombe) Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC004202 Gene Symbol: Chk1 Gene ID (NCBI): 1111 RRID: AB_3670463 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O14757 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.