Proteintech, 80064-2-RR, Phospho-MEK6 (Ser207) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MEK6 (Ser207) (80064-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-MEK6 (Ser207) in WB, ELISA applications with reactivity to human, mouse samples 80064-2-RR targets Phospho-MEK6 (Ser207) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: UV treated HEK-293 cells, HeLa cells, UV NIH/3T3 cells, UV treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). Because the polypeptide sequence near the Ser218 site of MEK3 is very similar to the Ser207 site of MEK6, this antibody also detects the phosphorylated at Ser218 of the MEK3, which is located at Ser189 in the isoform2 of MEK3. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase 6 Calculated Molecular Weight: 334 aa, 37 kDa Observed Molecular Weight: 38 kDa, 40kDa GenBank Accession Number: BC012009 Gene Symbol: MEK6 Gene ID (NCBI): 5608 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P52564 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.