Proteintech, 80080-2-RR, Phospho-PRKD1 (Ser916) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PRKD1 (Ser916) (80080-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-PRKD1 (Ser916) in WB, ELISA applications with reactivity to human, mouse samples 80080-2-RR targets Phospho-PRKD1 (Ser916) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein kinase D1 Calculated Molecular Weight: 102 kDa Observed Molecular Weight: 115 kDa GenBank Accession Number: NM_001330069 Gene Symbol: PRKD1 Gene ID (NCBI): 5587 RRID: AB_3670465 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q15139 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.