Proteintech, 80103-2-RR, Phospho-GRB10 (Ser476) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-GRB10 (Ser476) (80103-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-GRB10 (Ser476) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80103-2-RR targets Phospho-GRB10 (Ser476) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells Positive FC (Intra) detected in: λ phosphatase treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: growth factor receptor-bound protein 10 Calculated Molecular Weight: 536aa,61 kDa; 594aa,67 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC024285 Gene Symbol: GRB10 Gene ID (NCBI): 2887 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13322 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.