Proteintech, 80130-2-RR, Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-S6 Ribosomal protein (Ser235/236) (80130-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PDGF treated HEK-293 cells, PDGF treated C6 cells, PDGF treated NIH/3T3 cells Positive IF/ICC detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells Positive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 Observed Molecular Weight: 35 kDa GenBank Accession Number: NM_001010 Gene Symbol: RPS6 Gene ID (NCBI): 6194 RRID: AB_3670467 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P62753 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.