Proteintech, 80138-2-RR, Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT5A (Ser726)/STAT5B (Ser731) (80138-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 80138-2-RR targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: GM-CSF treated TF-1 cells Positive IF/ICC detected in: GM-CSF treated TF-1 cells Positive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated TF-1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318) Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 5A Calculated Molecular Weight: 794 aa, 92 kDa Observed Molecular Weight: 90-95 kDa GenBank Accession Number: BC027036 Gene Symbol: STAT5A Gene ID (NCBI): 6776 RRID: AB_3670468 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P42229 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.