Proteintech, 80144-2-RR, Phospho-SMAD9 (Ser465/467) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-SMAD9 (Ser465/467) (80144-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-SMAD9 (Ser465/467) in WB, ELISA, ChIP-qPCR applications with reactivity to human samples 80144-2-RR targets Phospho-SMAD9 (Ser465/467) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BMP2 treated HeLa cells Positive ChIP-qPCR detected in: BMP2 (50 ng/mL) for 1 h HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein. The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463/465. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SMAD family member 9 Calculated Molecular Weight: 430 aa, 49 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC011559 Gene Symbol: SMAD9 Gene ID (NCBI): 4093 RRID: AB_3670469 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O15198 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.