Proteintech, 80199-2-RR, Phospho-STAT3 (Ser727) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT3 (Ser727) (80199-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT3 (Ser727) in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples 80199-2-RR targets Phospho-STAT3 (Ser727) in WB, IHC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive FC (Intra) detected in: Calyculin A treated HEK-293T cells Positive ChIP-qPCR detected in: IL-6 (100ng/mL,30min) HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 3 (acute-phase response factor) Calculated Molecular Weight: 770 aa, 88 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC000627 Gene Symbol: STAT3 Gene ID (NCBI): 6774 RRID: AB_3670470 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P40763 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.