Proteintech, 80281-2-RR, Phospho-ACC1 (Ser79) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ACC1 (Ser79) (80281-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-ACC1 (Ser79) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 80281-2-RR targets Phospho-ACC1 (Ser79) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: λ phosphatase treated NIH/3T3 cells, NIH/3T3 cells Positive IF/ICC detected in: Calyculin A treated HeLa cells Positive FC (Intra) detected in: Calyculin A treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537) Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: acetyl-Coenzyme A carboxylase alpha Calculated Molecular Weight: 2383 aa, 275 kDa Observed Molecular Weight: 250-270 kDa GenBank Accession Number: BC137287 Gene Symbol: Acetyl-CoA Carboxylase 1 Gene ID (NCBI): 31 RRID: AB_3670473 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q13085 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.