Proteintech, 80294-2-RR, Phospho-P62/SQSTM1 (Ser349) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-P62/SQSTM1 (Ser349) (80294-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-P62/SQSTM1 (Ser349) in WB, ELISA applications with reactivity to human, mouse samples 80294-2-RR targets Phospho-P62/SQSTM1 (Ser349) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: sodium arsenite treated HEK-293 cells, MG132 treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: sequestosome 1 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC017222 Gene Symbol: P62/SQSTM1 Gene ID (NCBI): 8878 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13501 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.