Proteintech, 80301-1-RR, HSP90 Recombinant monoclonal antibody

Size: 20ul / 100ul The HSP90 (80301-1-RR) by Proteintech is a Recombinant antibody targeting HSP90 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, zebrafish samples 80301-1-RR targets HSP90 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples. Tested Applications Positive WB detected in: LNCaP cells, zebrafish tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, HSC-T6 cells, PC-12 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them. Specification Tested Reactivity: human, mouse, rat, zebrafish Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1947 Product name: Recombinant human HSP90AB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 330-710 aa of BC014485 Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP Predict reactive species Full Name: heat shock protein 90kDa alpha (cytosolic), class B member 1 Calculated Molecular Weight: 723 aa, 83 kDa Observed Molecular Weight: 83-90 kDa GenBank Accession Number: BC014485 Gene Symbol: HSP90AB1 Gene ID (NCBI): 3326 RRID: AB_2918885 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08238 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.