Proteintech, 80331-1-RR, JAK3 Recombinant monoclonal antibody

Size: 20ul / 100ul The JAK3 (80331-1-RR) by Proteintech is a Recombinant antibody targeting JAK3 in WB, IHC, IP, ELISA applications with reactivity to human samples 80331-1-RR targets JAK3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339) Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Janus kinase 3 (a protein tyrosine kinase, leukocyte) Calculated Molecular Weight: 1124 aa, 125 kDa Observed Molecular Weight: 125 kDa GenBank Accession Number: BC028068 Gene Symbol: JAK3 Gene ID (NCBI): 3718 RRID: AB_2918888 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P52333 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.