Proteintech, 80351-2-RR, Phospho-eEF2 (Thr57) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-eEF2 (Thr57) (80351-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-eEF2 (Thr57) in WB, FC (Intra), ELISA applications with reactivity to human samples 80351-2-RR targets Phospho-eEF2 (Thr57) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive FC (Intra) detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information eEF2 (Eukaryotic Elongation Factor 2) is a key component in the process of protein synthesis in eukaryotic cells. It is a GTP-binding protein that plays a crucial role in the elongation phase of translation. Specifically, eEF2 facilitates the movement of the ribosome along the mRNA, allowing the addition of amino acids to the growing polypeptide chain. This elongation factor is essential for efficient and accurate protein synthesis. Interestingly, eEF2 phosphorylation can either be increased or decreased depending on the stimulus used in learning or synaptic plasticity paradigms. Examining the specific contributions of increases and decreases in eEF2 phosphorylation to learning and memory is difficult. (PMID: 25762683) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation elongation factor 2 Calculated Molecular Weight: 858 aa, 95 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC126259 Gene Symbol: EEF2 Gene ID (NCBI): 29904 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13639 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.