Proteintech, 80351-3-RR, Phospho-eEF2K (Ser366) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-eEF2K (Ser366) (80351-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-eEF2K (Ser366) in WB, ELISA applications with reactivity to human samples 80351-3-RR targets Phospho-eEF2K (Ser366) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Eukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic elongation factor-2 kinase Calculated Molecular Weight: 725 aa, 100 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC032665 Gene Symbol: EEF2K Gene ID (NCBI): 29904 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O00418 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.