Proteintech, 80683-7-RR, Phospho-STAT1 (Ser727) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT1 (Ser727) (80683-7-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT1 (Ser727) in WB, ELISA, ChIP-qPCR applications with reactivity to human samples 80683-7-RR targets Phospho-STAT1 (Ser727) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IFN gamma treated HeLa cells Positive ChIP-qPCR detected in: IFN-γ (50 ng/ml, 30 min) HT-1080 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant duringlipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 1, 91kDa Calculated Molecular Weight: 83 kDa Observed Molecular Weight: 84-91 kDa GenBank Accession Number: BC002704 Gene Symbol: STAT1 Gene ID (NCBI): 6772 RRID: AB_3670483 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P42224 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.