Proteintech, 80706-3-RR, Phospho-c-SRC (Ser17) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-c-SRC (Ser17) (80706-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-c-SRC (Ser17) in WB, FC (Intra), ELISA applications with reactivity to human samples 80706-3-RR targets Phospho-c-SRC (Ser17) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293 cells Positive FC (Intra) detected in: λ phosphatase treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. The phosphorylation of c-SRC at Ser17 is essential for its activation. This phosphorylation event is often mediated by protein kinase A (PKA) and is crucial for downstream signaling events. (PMID: 23360994) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC011566 Gene Symbol: SRC Gene ID (NCBI): 6714 ENSEMBL Gene ID: ENSG00000197122 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P12931 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.