Proteintech, 81137-2-RR, AQP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The AQP1 (81137-2-RR) by Proteintech is a Recombinant antibody targeting AQP1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 81137-2-RR targets AQP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, rat kidney tissue Positive IHC detected in: mouse kidney tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 35-50 kDa. (PMID:20965731,16508653, 1530176). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag14093 Product name: Recombinant human AQP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 220-269 aa of BC022486 Sequence: GALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK Predict reactive species Full Name: aquaporin 1 (Colton blood group) Calculated Molecular Weight: 269 aa, 29 kDa Observed Molecular Weight: 25-28 kDa, 35-50 kDa GenBank Accession Number: BC022486 Gene Symbol: AQP1 Gene ID (NCBI): 358 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P29972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.