Proteintech, 81180-2-RR, Phospho-Paxillin (Tyr118) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Paxillin (Tyr118) (81180-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-Paxillin (Tyr118) in WB, IF/ICC, ELISA applications with reactivity to human samples 81180-2-RR targets Phospho-Paxillin (Tyr118) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: pervanadate treated HeLa cells Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: paxillin Calculated Molecular Weight: 591 aa, 65 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC136787 Gene Symbol: Paxillin Gene ID (NCBI): 5829 RRID: AB_3670493 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P49023 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.