Proteintech, 81251-2-RR, Phospho-PERK/EIF2AK3 (Ser719) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PERK/EIF2AK3 (Ser719) (81251-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-PERK/EIF2AK3 (Ser719) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 81251-2-RR targets Phospho-PERK/EIF2AK3 (Ser719) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells Positive FC (Intra) detected in: λ phosphatase treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 Calculated Molecular Weight: 1116 aa, 125 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC126354 Gene Symbol: PERK Gene ID (NCBI): 9451 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NZJ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.