Proteintech, 81609-1-RR, DMT1 Recombinant monoclonal antibody

Size: 20ul / 100ul The DMT1 (81609-1-RR) by Proteintech is a Recombinant antibody targeting DMT1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 81609-1-RR targets DMT1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, HuH-7 cells, Caco-2 cells, Neuro-2a cells, HSC-T6 cells Positive IP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag14314 Product name: Recombinant human SLC11A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-80 aa of BC002592 Sequence: MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKLWAFTGPGFLM Predict reactive species Full Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 Calculated Molecular Weight: 568 aa, 62 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC002592 Gene Symbol: DMT1 Gene ID (NCBI): 4891 RRID: AB_2935564 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P49281 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.