Proteintech, 81793-2-RR, CD71 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD71 (81793-2-RR) by Proteintech is a Recombinant antibody targeting CD71 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 81793-2-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-12 cells, DU 145 cells, HeLa cells, Raji cells, PC-3 cells, HT-1080 cells, K-562 cells, NIH/3T3 cells Positive IF/ICC detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0123 Product name: Recombinant human CD71 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 513-757 aa of BC001188 Sequence: VKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDID Predict reactive species Full Name: transferrin receptor (p90, CD71) Calculated Molecular Weight: 85 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC001188 Gene Symbol: CD71 Gene ID (NCBI): 7037 ENSEMBL Gene ID: ENSG00000072274 RRID: AB_3670505 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P02786 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.