Proteintech, 81854-1-RR, UBE2S Recombinant monoclonal antibody

Size: 20ul / 100ul The UBE2S (81854-1-RR) by Proteintech is a Recombinant antibody targeting UBE2S in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 81854-1-RR targets UBE2S in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, K-562 cells, NIH/3T3 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NCCIT cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5264 Product name: Recombinant human UBE2S protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC066948 Sequence: MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHLNVGANGEICVNVLKRDWTAELGIRHVLLTIRCLLIHPNPESALNEEAGRLLLENSEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTAASSTDPGAPGGLGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL Predict reactive species Full Name: ubiquitin-conjugating enzyme E2S Calculated Molecular Weight: 222 aa, 24 kDa Observed Molecular Weight: 28 kDa GenBank Accession Number: BC066948 Gene Symbol: UBE2S Gene ID (NCBI): 27338 RRID: AB_2935586 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16763 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.