Proteintech, 82524-2-RR, PKD2 Recombinant monoclonal antibody

Size: 20ul / 100ul The PKD2 (82524-2-RR) by Proteintech is a Recombinant antibody targeting PKD2 in WB, ELISA applications with reactivity to human samples 82524-2-RR targets PKD2 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Caki-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polycystic kidney disease 2 (autosomal dominant) Calculated Molecular Weight: 968 aa, 110 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC112263 Gene Symbol: PKD2 Gene ID (NCBI): 5311 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13563 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.