Proteintech, 82623-1-RR, GCLM Recombinant monoclonal antibody

Size: 20ul / 100ul The GCLM (82623-1-RR) by Proteintech is a Recombinant antibody targeting GCLM in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 82623-1-RR targets GCLM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, RAW 264.7 cells, HEK-293 cells, A431 cells, Jurkat cells, K-562 cells, HSC-T6 cells Positive IHC detected in: mouse brain tissue, human ovarian cancer, human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5497 Product name: Recombinant human GCLM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-274 aa of BC041809 Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS Predict reactive species Full Name: glutamate-cysteine ligase, modifier subunit Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC041809 Gene Symbol: GCLM Gene ID (NCBI): 2730 RRID: AB_3086493 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P48507 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.