Proteintech, 82805-2-RR, GRP94 Recombinant monoclonal antibody

Size: 20ul / 100ul The GRP94 (82805-2-RR) by Proteintech is a Recombinant antibody targeting GRP94 in WB, IHC, ELISA applications with reactivity to human samples 82805-2-RR targets GRP94 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells Positive IHC detected in: human breast cancer tissue, human lung cancer tissue, human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6400 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6344 Product name: Recombinant human GRP94 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 455-803 aa of BC066656 Sequence: KLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL Predict reactive species Full Name: heat shock protein 90kDa beta (Grp94), member 1 Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 95-100 kDa GenBank Accession Number: BC066656 Gene Symbol: GRP94 Gene ID (NCBI): 7184 RRID: AB_3670558 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P14625 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.